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The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.

The mammalian target of rapamycin (mTOR) controls multiple cellular functions in response to amino acids and growth factors, in part by regulating the phosphorylation of p70 S6 kinase (p70S6k) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1). Raptor (regulatory associated protein of mTOR) is a recently identified mTOR binding partner that also binds p70S6k and 4E-BP1 and is essential for TOR signaling in vivo. Herein we demonstrate that raptor binds to p70S6k and 4E-BP1 through their respective TOS (conserved TOR signaling) motifs to be required for amino acid- and mTOR-dependent regulation of these mTOR substrates in vivo. A point mutation of the TOS motif also eliminates all in vitro mTOR-catalyzed 4E-BP1 phosphorylation and abolishes the raptor-dependent component of mTOR-catalyzed p70S6k phosphorylation in vitro. Raptor appears to serve as an mTOR scaffold protein, the binding of which to the TOS motif of mTOR substrates is necessary for effective mTOR-catalyzed phosphorylation in vivo and perhaps for conferring their sensitivity to rapamycin and amino acid sufficiency.

Pubmed ID: 12604610

Authors

  • Nojima H
  • Tokunaga C
  • Eguchi S
  • Oshiro N
  • Hidayat S
  • Yoshino K
  • Hara K
  • Tanaka N
  • Avruch J
  • Yonezawa K

Journal

The Journal of biological chemistry

Publication Data

May 2, 2003

Associated Grants

  • Agency: NCI NIH HHS, Id: CA 73818
  • Agency: NIDDK NIH HHS, Id: DK 17776

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Motifs
  • Carrier Proteins
  • Cells, Cultured
  • Humans
  • Phosphoproteins
  • Phosphorylation
  • Protein Kinases
  • Proteins
  • Ribosomal Protein S6 Kinases, 70-kDa
  • TOR Serine-Threonine Kinases