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Naf1 p is a box H/ACA snoRNP assembly factor.

Box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs) contain four essential proteins, Cbf5p, Gar1p, Nhp2p, and Nop10p, each of which, with the exception of Gar1p, is required for box H/ACA snoRNA accumulation. Database searches identified a novel essential protein, which we termed Naf1p, with a region of homology to the RNA-binding domain of Gar1p and other features in common with hnRNP-like proteins. Naf1p is localized to the nucleus and is not a stable component of the H/ACA snoRNPs, but it is required for the accumulation of all box H/ACA snoRNAs. This requirement is not at the level of snoRNA transcription initiation or termination. Naf1 p shows in vitro RNA-binding activity and also binds directly to Cbf5p and Nhp2p. Naf1p was shown to bind to the CTD in vivo in a two-hybrid assay, and the phosphorylated CTD, but not the nonphosphorylated CTD, was shown to precipitate tagged Naf1p from a cell lysate. We propose that Naf1 p is recruited to the CTD of RNA polymerase II and binds to nascent box H/ACA snoRNAs promoting snoRNP assembly.

Pubmed ID: 12515383

Authors

  • Fatica A
  • Dlakić M
  • Tollervey D

Journal

RNA (New York, N.Y.)

Publication Data

December 7, 2002

Associated Grants

None

Mesh Terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Binding Sites
  • Cell Nucleus
  • Fungal Proteins
  • Hydro-Lyases
  • Microtubule-Associated Proteins
  • Molecular Sequence Data
  • Nuclear Proteins
  • RNA Polymerase II
  • RNA Processing, Post-Transcriptional
  • RNA, Ribosomal
  • RNA, Small Nucleolar
  • RNA-Binding Proteins
  • Recombinant Proteins
  • Ribonucleoproteins, Small Nuclear
  • Ribonucleoproteins, Small Nucleolar
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Yeasts