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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
The National Institutes of Health Mammalian Gene Collection (MGC) Program is a multiinstitutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and mouse gene. ESTs were generated from libraries enriched for full-length cDNAs and analyzed to identify candidate full-ORF clones, which then were sequenced to high accuracy. The MGC has currently sequenced and verified the full ORF for a nonredundant set of >9,000 human and >6,000 mouse genes. Candidate full-ORF clones for an additional 7,800 human and 3,500 mouse genes also have been identified. All MGC sequences and clones are available without restriction through public databases and clone distribution networks (see http:mgc.nci.nih.gov).
Pubmed ID: 12477932
Strausberg RL Feingold EA Grouse LH Derge JG Klausner RD Collins FS Wagner L Shenmen CM Schuler GD Altschul SF Zeeberg B Buetow KH Schaefer CF Bhat NK Hopkins RF Jordan H Moore T Max SI Wang J Hsieh F Diatchenko L Marusina K Farmer AA Rubin GM Hong L Stapleton M Soares MB Bonaldo MF Casavant TL Scheetz TE Brownstein MJ Usdin TB Toshiyuki S Carninci P Prange C Raha SS Loquellano NA Peters GJ Abramson RD Mullahy SJ Bosak SA McEwan PJ McKernan KJ Malek JA Gunaratne PH Richards S Worley KC Hale S Garcia AM Gay LJ Hulyk SW Villalon DK Muzny DM Sodergren EJ Lu X Gibbs RA Fahey J Helton E Ketteman M Madan A Rodrigues S Sanchez A Whiting M Madan A Young AC Shevchenko Y Bouffard GG Blakesley RW Touchman JW Green ED Dickson MC Rodriguez AC Grimwood J Schmutz J Myers RM Butterfield YS Krzywinski MI Skalska U Smailus DE Schnerch A Schein JE Jones SJ Marra MA Mammalian Gene Collection Program Team
Proceedings of the National Academy of Sciences of the United States of America
December 24, 2002
Agency: NHGRI NIH HHS, Id: U54 HG003273
Algorithms Animals DNA, Complementary Gene Library Humans Mice Open Reading Frames Sequence Analysis, DNA
CYP2B protein: Secondary metabolites biosynthesis, transport and catabolism ASRGL1 protein: Amino acid transport and metabolism Chromosome 14 open reading frame 149 CDNA FLJ23636 fis, clone CAS07176: Posttranslational modification, protein turnover, chaperones SHMT2 protein: Amino acid transport and metabolism KIAA0251 protein: Amino acid transport and metabolism Glutamate decarboxylase 1: Amino acid transport and metabolism Aminolevulinate, delta-, synthase 2: Coenzyme transport and metabolism Choline/ethanolaminephosphotransferase Glutathione peroxidase 4: Posttranslational modification, protein turnover, chaperones GAD1 protein: Amino acid transport and metabolism AMT protein: Amino acid transport and metabolism Oxidoreductase: Lipid transport and metabolism Hypothetical protein: Posttranslational modification, protein turnover, chaperones CDNA FLJ46802 fis, clone TRACH3032150, moderately similar to Cysteine sulfinic acid decarboxylase: Amino acid transport and metabolism 2-oxoglutarate and iron-dependent oxygenase domain containing 1 Sigma 1-type opioid receptor: Involved in C-8 sterol isomerase activity SHMT2 protein: Amino acid transport and metabolism