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The core-binding factor beta subunit is required for bone formation and hematopoietic maturation.

Core-binding factor beta (Cbfbeta) is the common non-DNA-binding subunit of the Cbf family of heterodimeric transcription factors. Mice deficient in Cbfbeta have a severe block in fetal liver hematopoiesis at the stage of hematopoietic stem cell (HSC) emergence. Here we show that by providing Cbfbeta function in endothelial cells and hematopoietic progenitors we can rescue fetal liver hematopoiesis in Cbfbeta-deficient embryos. The rescued mice die at birth, however, with severe defects in skeletal development, though intramembranous ossification occurs to some extent. Fetal liver hematopoiesis is restored at embryonic day (E) 12.5, but by E17.5 significant impairments in lymphopoiesis and myelopoiesis are observed. Thus, we conclude that the Cbfbeta subunit is required for HSC emergence, bone formation and normal differentiation of lymphoid and myeloid lineage cells.

Pubmed ID: 12434155

Authors

  • Miller J
  • Horner A
  • Stacy T
  • Lowrey C
  • Lian JB
  • Stein G
  • Nuckolls GH
  • Speck NA

Journal

Nature genetics

Publication Data

December 28, 2002

Associated Grants

None

Mesh Terms

  • Animals
  • Cell Differentiation
  • Cloning, Molecular
  • DNA-Binding Proteins
  • Embryonic and Fetal Development
  • Endothelium, Vascular
  • Fetus
  • Gene Expression Regulation, Developmental
  • Genes, Lethal
  • Genotype
  • Green Fluorescent Proteins
  • Hematopoiesis
  • Hematopoietic Stem Cells
  • Liver
  • Luminescent Proteins
  • Mice
  • Mice, Transgenic
  • Osteoblasts
  • Osteogenesis
  • Recombinant Fusion Proteins
  • Transcription Factor AP-2
  • Transcription Factors