SUMO-1 protease-1 regulates gene transcription through PML.
During a screen to identify c-Jun activators, we isolated a cysteine protease, SuPr-1, that induced c-Jun-dependent transcription independently of c-Jun phosphorylation. SuPr-1 is a member of a new family of proteases that hydrolyze the ubiquitin-like modifier, SUMO-1. SuPr-1 hydrolyzed SUMO-1-modified forms of the promyelocytic leukemia gene product, PML, and altered the subcellular distribution of PML in nuclear PODs (PML oncogenic domains). SuPr-1 also altered the distribution of other nuclear POD-associated proteins, such as CBP and Daxx, that act as transcriptional regulators. SuPr-1 action on transcription was enhanced by PML, and SuPr-1 failed to activate transcription in PML-deficient fibroblasts. Our studies establish an important role for SUMO proteases in transcription.
Pubmed ID: 12419228 RIS Download
Amino Acid Sequence | Animals | Base Sequence | Cell Line | Cell Nucleus Structures | Cysteine Endopeptidases | HeLa Cells | Humans | Mice | Molecular Sequence Data | Mutation | Neoplasm Proteins | Nuclear Proteins | Phosphorylation | Promoter Regions, Genetic | Promyelocytic Leukemia Protein | Protein Binding | Proto-Oncogene Proteins c-jun | Sequence Homology, Nucleic Acid | Substrate Specificity | Transcription Factors | Transcriptional Activation | Tumor Suppressor Proteins