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SUMO-1 protease-1 regulates gene transcription through PML.

During a screen to identify c-Jun activators, we isolated a cysteine protease, SuPr-1, that induced c-Jun-dependent transcription independently of c-Jun phosphorylation. SuPr-1 is a member of a new family of proteases that hydrolyze the ubiquitin-like modifier, SUMO-1. SuPr-1 hydrolyzed SUMO-1-modified forms of the promyelocytic leukemia gene product, PML, and altered the subcellular distribution of PML in nuclear PODs (PML oncogenic domains). SuPr-1 also altered the distribution of other nuclear POD-associated proteins, such as CBP and Daxx, that act as transcriptional regulators. SuPr-1 action on transcription was enhanced by PML, and SuPr-1 failed to activate transcription in PML-deficient fibroblasts. Our studies establish an important role for SUMO proteases in transcription.

Pubmed ID: 12419228

Authors

  • Best JL
  • Ganiatsas S
  • Agarwal S
  • Changou A
  • Salomoni P
  • Shirihai O
  • Meluh PB
  • Pandolfi PP
  • Zon LI

Journal

Molecular cell

Publication Data

October 6, 2002

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cell Nucleus Structures
  • Cysteine Endopeptidases
  • HeLa Cells
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Neoplasm Proteins
  • Nuclear Proteins
  • Phosphorylation
  • Promoter Regions, Genetic
  • Protein Binding
  • Proto-Oncogene Proteins c-jun
  • Sequence Homology, Nucleic Acid
  • Substrate Specificity
  • Transcription Factors
  • Transcriptional Activation
  • Tumor Suppressor Proteins