Potentiation of serum response factor activity by a family of myocardin-related transcription factors.
Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.
Pubmed ID: 12397177 RIS Download
Amino Acid Sequence | Animals | Base Sequence | Binding Sites | Blotting, Northern | Cloning, Molecular | DNA, Complementary | Expressed Sequence Tags | Gene Expression Regulation, Developmental | Glutathione Transferase | Humans | Mice | Molecular Sequence Data | Myocardium | Nuclear Proteins | Recombinant Fusion Proteins | Sequence Alignment | Sequence Homology, Amino Acid | Serum Response Factor | Trans-Activators | Transcription Factors | Xenopus