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MRG15, a novel chromodomain protein, is present in two distinct multiprotein complexes involved in transcriptional activation.

MRG15 is a novel chromodomain protein that is a member of a family of genes related to MORF4. MORF4 (mortality factor on chromosome 4) induces senescence in a subset of human tumor cell lines. Our previous results indicated that MRG15 (MORF-related gene on chromosome 15) could derepress the B-myb promoter by association with Rb. In this study, sucrose gradient analysis demonstrated that MRG15 was present in two distinct nuclear protein complexes, MAF1 (MRG15-associated factor 1) and MAF2. Rb was associated with MRG15 and PAM14 (a novel coil-coil protein) in MAF1, and a histone acetyl transferase, hMOF, was an MRG15 partner in MAF2. Analysis of deletion mutants of MRG15 indicated that the leucine zipper at the C-terminal region of MRG15 was important for the protein associations in MAF1 and that the N-terminal chromodomain was required for the assembly of the MAF2 protein complex. Consistent with these data was the fact that a histone acetyltransferase activity associated with MRG15 was lost when the chromodomain was deleted and that both mutant MRG15 proteins failed to activate the B-myb promoter. The various mechanisms by which MRG15 could activate gene transcription are discussed.

Pubmed ID: 12397079


  • Pardo PS
  • Leung JK
  • Lucchesi JC
  • Pereira-Smith OM


The Journal of biological chemistry

Publication Data

December 27, 2002

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM15691
  • Agency: NIA NIH HHS, Id: P01AG13663
  • Agency: NIA NIH HHS, Id: R37AG05333
  • Agency: NIA NIH HHS, Id: T32AG00183

Mesh Terms

  • Acetyltransferases
  • Base Sequence
  • Cell Nucleus
  • Chromosomal Proteins, Non-Histone
  • Chromosomes, Human, Pair 15
  • Cloning, Molecular
  • Genes, myb
  • Histone Acetyltransferases
  • Humans
  • Molecular Sequence Data
  • Nucleoproteins
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcriptional Activation
  • Tumor Cells, Cultured