• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


MRG15, a novel chromodomain protein, is present in two distinct multiprotein complexes involved in transcriptional activation.

MRG15 is a novel chromodomain protein that is a member of a family of genes related to MORF4. MORF4 (mortality factor on chromosome 4) induces senescence in a subset of human tumor cell lines. Our previous results indicated that MRG15 (MORF-related gene on chromosome 15) could derepress the B-myb promoter by association with Rb. In this study, sucrose gradient analysis demonstrated that MRG15 was present in two distinct nuclear protein complexes, MAF1 (MRG15-associated factor 1) and MAF2. Rb was associated with MRG15 and PAM14 (a novel coil-coil protein) in MAF1, and a histone acetyl transferase, hMOF, was an MRG15 partner in MAF2. Analysis of deletion mutants of MRG15 indicated that the leucine zipper at the C-terminal region of MRG15 was important for the protein associations in MAF1 and that the N-terminal chromodomain was required for the assembly of the MAF2 protein complex. Consistent with these data was the fact that a histone acetyltransferase activity associated with MRG15 was lost when the chromodomain was deleted and that both mutant MRG15 proteins failed to activate the B-myb promoter. The various mechanisms by which MRG15 could activate gene transcription are discussed.

Pubmed ID: 12397079


  • Pardo PS
  • Leung JK
  • Lucchesi JC
  • Pereira-Smith OM


The Journal of biological chemistry

Publication Data

December 27, 2002

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM15691
  • Agency: NIA NIH HHS, Id: P01AG13663
  • Agency: NIA NIH HHS, Id: R37AG05333
  • Agency: NIA NIH HHS, Id: T32AG00183

Mesh Terms

  • Acetyltransferases
  • Base Sequence
  • Cell Nucleus
  • Chromosomal Proteins, Non-Histone
  • Chromosomes, Human, Pair 15
  • Cloning, Molecular
  • Genes, myb
  • Histone Acetyltransferases
  • Humans
  • Molecular Sequence Data
  • Nucleoproteins
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcriptional Activation
  • Tumor Cells, Cultured