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Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells.

We have generated transgenic mice that express green fluorescent protein (GFP) under the control of the mouse insulin I gene promoter (MIP). The MIP-GFP mice develop normally and are indistinguishable from control animals with respect to glucose tolerance and pancreatic insulin content. Histological studies showed that the MIP-GFP mice had normal islet architecture with coexpression of insulin and GFP in the beta-cells of all islets. We observed GFP expression in islets from embryonic day E13.5 through adulthood. Studies of beta-cell function revealed no difference in glucose-induced intracellular calcium mobilization between islets from transgenic and control animals. We prepared single-cell suspensions from both isolated islets and whole pancreas from MIP-GFP-transgenic mice and sorted the beta-cells by fluorescence-activated cell sorting based on their green fluorescence. These studies showed that 2.4 +/- 0.2% (n = 6) of the cells in the pancreas of newborn (P1) and 0.9 +/- 0.1% (n = 5) of 8-wk-old mice were beta-cells. The MIP-GFP-transgenic mouse may be a useful tool for studying beta-cell biology in normal and diabetic animals.

Pubmed ID: 12388130


  • Hara M
  • Wang X
  • Kawamura T
  • Bindokas VP
  • Dizon RF
  • Alcoser SY
  • Magnuson MA
  • Bell GI


American journal of physiology. Endocrinology and metabolism

Publication Data

January 17, 2003

Associated Grants

  • Agency: NIDDK NIH HHS, Id: DK-20595
  • Agency: NIDDK NIH HHS, Id: DK-44840
  • Agency: NIDDK NIH HHS, Id: DK-61245

Mesh Terms

  • Aging
  • Animals
  • Blotting, Western
  • Calcium
  • Cell Count
  • Female
  • Flow Cytometry
  • Gene Expression
  • Glucose
  • Glucose Tolerance Test
  • Green Fluorescent Proteins
  • Insulin
  • Islets of Langerhans
  • Luminescent Proteins
  • Male
  • Mice
  • Mice, Transgenic
  • Pancreas
  • Promoter Regions, Genetic