We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Requirement for Foxd3 in maintaining pluripotent cells of the early mouse embryo.

Genes & development | Oct 15, 2002

Critical to our understanding of the developmental potential of stem cells and subsequent control of their differentiation in vitro and in vivo is a thorough understanding of the genes that control stem cell fate. Here, we report that Foxd3, a member of the forkhead family of transcriptional regulators, is required for maintenance of embryonic cells of the early mouse embryo. Foxd3-/- embryos die after implantation at approximately 6.5 days postcoitum with a loss of epiblast cells, expansion of proximal extraembryonic tissues, and a distal, mislocalized anterior organizing center. Moreover, it has not been possible to establish Foxd3-/- ES cell lines or to generate Foxd3-/- teratocarcinomas. Chimera analysis reveals that Foxd3 function is required in the epiblast and that Foxd3-/- embryos can be rescued by a small number of wild-type cells. Foxd3-/- mutant blastocysts appear morphologically normal and express Oct4, Sox2, and Fgf4, but when placed in vitro the inner cell mass initially proliferates and then fails to expand even when Fgf4 is added. These results establish Foxd3 as a factor required for the maintenance of progenitor cells in the mammalian embryo.

Pubmed ID: 12381664 RIS Download

Mesh terms: Animals | Blastocyst | Cell Differentiation | Cells, Cultured | DNA Primers | DNA-Binding Proteins | Embryonic and Fetal Development | Female | Fibroblast Growth Factor 4 | Fibroblast Growth Factors | Forkhead Transcription Factors | Gene Expression Regulation, Developmental | Helix-Turn-Helix Motifs | In Situ Hybridization | In Vitro Techniques | Male | Mice | Mice, Inbred C57BL | Mice, Mutant Strains | Octamer Transcription Factor-3 | Pluripotent Stem Cells | Proto-Oncogene Proteins | RNA, Messenger | Repressor Proteins | Reverse Transcriptase Polymerase Chain Reaction | Signal Transduction | Transcription Factors

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NICHD NIH HHS, Id: R01HD36720
  • Agency: NICHD NIH HHS, Id: R01 HD036720
  • Agency: NHLBI NIH HHS, Id: F32HL10421
  • Agency: NIGMS NIH HHS, Id: R01 GM064768
  • Agency: NHLBI NIH HHS, Id: F32 HL010421
  • Agency: NIGMS NIH HHS, Id: R01GM64768

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.