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Rab11-FIP2, an adaptor protein connecting cellular components involved in internalization and recycling of epidermal growth factor receptors.

Rab11-FIP2 is a member of a newly identified family of Rab11-binding proteins that have been implicated in the function of recycling endosomes. Here we show that Rab11-FIP2 may also be involved with the process of receptor-mediated endocytosis. First we demonstrate that Rab11-FIP2 contains an NPF motif that allows it to bind Reps1, a member of a family of EH domain proteins involved in endocytosis. We also show that Rab11-FIP2 associates with the alpha-adaptin subunit of AP-2 complexes, which are known to recruit receptors into clathrin-coated vesicles. Finally, we find that overexpression of Rab11-FIP2 suppresses the internalization of epidermal growth factor receptors, but not transferrin receptors, through binding sites that promote complex formation with Rab11, Reps1, and alpha-adaptin. These findings suggest that Rab11-FIP2 may participate in the coupling of receptor-mediated endocytosis to the subsequent sorting of receptor-containing vesicles in endosomes.

Pubmed ID: 12364336


  • Cullis DN
  • Philip B
  • Baleja JD
  • Feig LA


The Journal of biological chemistry

Publication Data

December 20, 2002

Associated Grants

  • Agency: PHS HHS, Id: NIH R01 47717

Mesh Terms

  • 3T3 Cells
  • Adaptor Protein Complex 2
  • Adaptor Protein Complex alpha Subunits
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • COS Cells
  • Carrier Proteins
  • Endocytosis
  • Endosomes
  • Glutathione Transferase
  • Membrane Proteins
  • Mice
  • Models, Biological
  • Molecular Sequence Data
  • Peptides
  • Plasmids
  • Precipitin Tests
  • Protein Binding
  • Protein Structure, Tertiary
  • Receptor, Epidermal Growth Factor
  • Sequence Homology, Amino Acid
  • Software
  • Spectrometry, Fluorescence
  • Transfection
  • Two-Hybrid System Techniques
  • rab GTP-Binding Proteins