• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells.

Centrosome duplication and separation are linked inextricably to certain cell cycle events, in particular activation of cyclin-dependent kinases (CDKs). However, relatively few CDK targets driving these events have been uncovered. Here, we have performed a screen for CDK substrates and have isolated a target, CP110, which is phosphorylated by CDKs in vitro and in vivo. Human CP110 localizes to centrosomes. Its expression is strongly induced at the G1-to-S phase transition, coincident with the initiation of centrosome duplication. RNAi-mediated depletion of CP110 indicates that this protein plays an essential role in centrosome duplication. Long-term disruption of CP110 phosphorylation leads to unscheduled centrosome separation and overt polyploidy. Our data suggest that CP110 is a physiological centrosomal CDK target that promotes centrosome duplication, and its deregulation may contribute to genomic instability.

Pubmed ID: 12361598


  • Chen Z
  • Indjeian VB
  • McManus M
  • Wang L
  • Dynlacht BD


Developmental cell

Publication Data

September 3, 2002

Associated Grants


Mesh Terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Cell Cycle Proteins
  • Centrosome
  • Cloning, Molecular
  • Cyclin-Dependent Kinases
  • DNA, Complementary
  • G1 Phase
  • Gene Expression Regulation
  • HeLa Cells
  • Humans
  • In Situ Hybridization, Fluorescence
  • Microtubule-Associated Proteins
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Phosphoproteins
  • Phosphorylation
  • Polyploidy
  • RNA, Small Interfering
  • Recombinant Proteins
  • S Phase
  • Sensitivity and Specificity
  • Sequence Alignment
  • Substrate Specificity
  • Tumor Cells, Cultured