Our hosting provider will be undergoing maintenance on Tuesday, August 30 between 8am and 5pm PDT. SciCrunch services may be offline during the maintenance.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V.

Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth and cytokinesis. Mlc1p has been crystallized in complexes with four different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4 and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple anomalous dispersion (MAD) experiments based on selenomethionine derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model.

Pubmed ID: 12351846

Authors

  • Terrak M
  • Otterbein LR
  • Wu G
  • Palecanda LA
  • Lu RC
  • Dominguez R

Journal

Acta crystallographica. Section D, Biological crystallography

Publication Data

October 27, 2002

Associated Grants

  • Agency: NIAMS NIH HHS, Id: R01 AR046524
  • Agency: NIAMS NIH HHS, Id: R01 AR46524
  • Agency: NCRR NIH HHS, Id: RR-01646
  • Agency: NCRR NIH HHS, Id: RR07707

Mesh Terms

  • Amino Acid Sequence
  • Binding Sites
  • Cell Division
  • Cloning, Molecular
  • Escherichia coli
  • Models, Molecular
  • Molecular Sequence Data
  • Myosin Light Chains
  • Myosin Type V
  • Protein Conformation
  • Recombinant Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • X-Ray Diffraction