• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

A targeted RNAi screen for genes involved in chromosome morphogenesis and nuclear organization in the Caenorhabditis elegans germline.

We have implemented a functional genomics strategy to identify genes involved in chromosome morphogenesis and nuclear organization during meiotic prophase in the Caenorhabditis elegans germline. This approach took advantage of a gene-expression survey that used DNA microarray technology to identify genes preferentially expressed in the germline. We defined a subset of 192 germline-enriched genes whose expression profiles were similar to those of previously identified meiosis genes and designed a screen to identify genes for which inhibition by RNA interference (RNAi) elicited defects in function or development of the germline. We obtained strong germline phenotypes for 27% of the genes tested, indicating that this targeted approach greatly enriched for genes that function in the germline. In addition to genes involved in key meiotic prophase events, we identified genes involved in meiotic progression, germline proliferation, and chromosome organization and/or segregation during mitotic growth.

Pubmed ID: 12242227

Authors

  • Colai├ícovo MP
  • Stanfield GM
  • Reddy KC
  • Reinke V
  • Kim SK
  • Villeneuve AM

Journal

Genetics

Publication Data

September 20, 2002

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM-53804

Mesh Terms

  • Animals
  • Base Sequence
  • Caenorhabditis elegans
  • DNA Primers
  • Gene Expression
  • Germ Cells
  • Meiosis
  • Mitosis
  • Morphogenesis
  • RNA
  • Reproducibility of Results