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A cell type-specific allele of the POU gene Oct-6 reveals Schwann cell autonomous function in nerve development and regeneration.

The EMBO journal | Sep 2, 2002

http://www.ncbi.nlm.nih.gov/pubmed/12198163

While an important role for the POU domain transcription factor Oct-6 in the developing peripheral nerve has been well established, studies into its exact role in nerve development and regeneration have been hampered by the high mortality rate of newborn Oct-6 mutant animals. In this study we have generated a Schwann cell-specific Oct-6 allele through deletion of the Schwann cell-specific enhancer element (SCE) in the Oct-6 locus. Analysis of mice homozygous for this allele (deltaSCE allele) reveals that rate-limiting levels of Oct-6 in Schwann cells are dependent on the SCE and that this element does not contribute to Oct-6 regulation in other cell types. We demonstrate a Schwann cell autonomous function for Oct-6 during nerve development as well as in regenerating nerve. Additionally, we show that Krox-20, an important regulatory target of Oct-6 in Schwann cells, is activated, with delayed kinetics, through an Oct-6-independent mechanism in these mice.

Pubmed ID: 12198163 RIS Download

Mesh terms: Alleles | Animals | Cell Lineage | Chimera | Crosses, Genetic | DNA-Binding Proteins | Early Growth Response Protein 2 | Enhancer Elements, Genetic | Female | Gene Targeting | Male | Mice | Myelin Sheath | Nerve Regeneration | Nerve Tissue Proteins | Octamer Transcription Factor-6 | Organ Specificity | Peripheral Nerves | Schwann Cells | Sequence Deletion | Transcription Factors

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