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Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body.

Sorting of ubiquitinated endosomal membrane proteins into the MVB pathway is executed by the class E Vps protein complexes ESCRT-I, -II, and -III, and the AAA-type ATPase Vps4. This study characterizes ESCRT-II, a soluble approximately 155 kDa protein complex formed by the class E Vps proteins Vps22, Vps25, and Vps36. This protein complex transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo. We propose that the ESCRT complexes perform a coordinated cascade of events to select and sort MVB cargoes for delivery to the lumen of the vacuole/lysosome.

Pubmed ID: 12194858

Authors

  • Babst M
  • Katzmann DJ
  • Snyder WB
  • Wendland B
  • Emr SD

Journal

Developmental cell

Publication Data

August 26, 2002

Associated Grants

  • Agency: NCI NIH HHS, Id: CA58689

Mesh Terms

  • Carrier Proteins
  • Cell Membrane
  • Endosomes
  • Eukaryotic Cells
  • Fungal Proteins
  • Lysosomes
  • Macromolecular Substances
  • Membrane Proteins
  • Protein Binding
  • Protein Transport
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transport Vesicles
  • Ubiquitin