Literature search services are currently unavailable. During our hosting provider's UPS upgrade we experienced a hardware failure and are currently working to resolve the issue.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin.

We report the identification and expression pattern of a full-length human cDNA and a partial mouse cDNA encoding cyclin B3. Cyclin B3 (CCNB3) is conserved from Caenorhabditis elegans to Homo sapiens and has an undefined meiotic function in female, but not male Drosophila melanogaster. We show that H. sapiens cyclin B3 interacts with cdk2, is localized to the nucleus, and is degraded during anaphase entry after the degradation of cyclin B1. Degradation is dependent on sequences conserved in a destruction box motif. Overexpression of nondegradable cyclin B3 blocks the mitotic cell cycle in late anaphase, and at higher doses it can interfere with progression through G(1) and entry into S phase. H. sapiens cyclin B3 mRNA and protein are detected readily in developing germ cells in the human testis and not in any other tissue. The mouse cDNA has allowed us to further localize cyclin B3 mRNA to leptotene and zygotene spermatocytes. The expression pattern of mammalian cyclin B3 suggests that it may be important for events occurring in early meiotic prophase I.

Pubmed ID: 12185076


  • Nguyen TB
  • Manova K
  • Capodieci P
  • Lindon C
  • Bottega S
  • Wang XY
  • Refik-Rogers J
  • Pines J
  • Wolgemuth DJ
  • Koff A


The Journal of biological chemistry

Publication Data

November 1, 2002

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM52597

Mesh Terms

  • Animals
  • Base Sequence
  • Cell Nucleus
  • Cyclin B
  • Cyclin B1
  • Cyclins
  • Drosophila Proteins
  • Female
  • Fetus
  • G2 Phase
  • HeLa Cells
  • Humans
  • Male
  • Meiosis
  • Mice
  • Mitosis
  • Molecular Sequence Data
  • Ovary
  • RNA, Messenger
  • Spermatogenesis
  • Testis