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Role of ANC-1 in tethering nuclei to the actin cytoskeleton.

Mutations in anc-1 (nuclear anchorage defective) disrupt the positioning of nuclei and mitochondria in Caenorhabditis elegans. ANC-1 is shown to consist of mostly coiled regions with a nuclear envelope localization domain (called the KASH domain) and an actin-binding domain; this structure was conserved with the Drosophila protein Msp-300 and the mammalian Syne proteins. Antibodies against ANC-1 localized cytoplasmically and were enriched at the nuclear periphery in an UNC-84-dependent manner. Overexpression of the KASH domain or the actin-binding domain caused a dominant negative anchorage defect. Thus, ANC-1 may connect nuclei to the cytoskeleton by interacting with UNC-84 at the nuclear envelope and with actin in the cytoplasm.

Pubmed ID: 12169658

Authors

  • Starr DA
  • Han M

Journal

Science (New York, N.Y.)

Publication Data

October 11, 2002

Associated Grants

None

Mesh Terms

  • Actins
  • Alleles
  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins
  • Cell Nucleus
  • Cytoplasm
  • Cytoskeleton
  • Genes, Helminth
  • Membrane Glycoproteins
  • Microfilament Proteins
  • Mitochondria
  • Mutation
  • Nuclear Envelope
  • Nuclear Proteins
  • Phenotype
  • Protein Binding
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins