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Role of ANC-1 in tethering nuclei to the actin cytoskeleton.

Science (New York, N.Y.) | Oct 11, 2002

http://www.ncbi.nlm.nih.gov/pubmed/12169658

Mutations in anc-1 (nuclear anchorage defective) disrupt the positioning of nuclei and mitochondria in Caenorhabditis elegans. ANC-1 is shown to consist of mostly coiled regions with a nuclear envelope localization domain (called the KASH domain) and an actin-binding domain; this structure was conserved with the Drosophila protein Msp-300 and the mammalian Syne proteins. Antibodies against ANC-1 localized cytoplasmically and were enriched at the nuclear periphery in an UNC-84-dependent manner. Overexpression of the KASH domain or the actin-binding domain caused a dominant negative anchorage defect. Thus, ANC-1 may connect nuclei to the cytoskeleton by interacting with UNC-84 at the nuclear envelope and with actin in the cytoplasm.

Pubmed ID: 12169658 RIS Download

Mesh terms: Actins | Alleles | Animals | Animals, Genetically Modified | Caenorhabditis elegans | Caenorhabditis elegans Proteins | Cell Nucleus | Cytoplasm | Cytoskeleton | Genes, Helminth | Membrane Glycoproteins | Microfilament Proteins | Mitochondria | Mutation | Nuclear Envelope | Nuclear Proteins | Phenotype | Protein Binding | Protein Structure, Tertiary | Recombinant Fusion Proteins