Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action.

Cell | Jul 26, 2002

http://www.ncbi.nlm.nih.gov/pubmed/12150926

mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

Pubmed ID: 12150926 RIS Download

Mesh terms: Adaptor Proteins, Signal Transducing | Animals | Base Sequence | Caenorhabditis elegans | Carrier Proteins | Cell Line | Cloning, Molecular | DNA, Complementary | Enzyme Activation | Gene Expression | Gene Silencing | HeLa Cells | Humans | Molecular Sequence Data | Phenotype | Phosphoproteins | Phosphorylation | Protein Binding | Protein Kinases | Proteins | RNA Cap Analogs | RNA, Small Interfering | RNA, Untranslated | Ribosomal Protein S6 Kinases | Sepharose | TOR Serine-Threonine Kinases

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: R01 CA73818B 06

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.