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Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex.

Mammalian Dock180 and ELMO proteins, and their homologues in Caenorhabditis elegans and Drosophila melanogaster, function as critical upstream regulators of Rac during development and cell migration. The mechanism by which Dock180 or ELMO mediates Rac activation is not understood. Here, we identify a domain within Dock180 (denoted Docker) that specifically recognizes nucleotide-free Rac and can mediate GTP loading of Rac in vitro. The Docker domain is conserved among known Dock180 family members in metazoans and in a yeast protein. In cells, binding of Dock180 to Rac alone is insufficient for GTP loading, and a Dock180 ELMO1 interaction is required. We can also detect a trimeric ELMO1 Dock180 Rac1 complex and ELMO augments the interaction between Dock180 and Rac. We propose that the Dock180 ELMO complex functions as an unconventional two-part exchange factor for Rac.

Pubmed ID: 12134158

Authors

  • Brugnera E
  • Haney L
  • Grimsley C
  • Lu M
  • Walk SF
  • Tosello-Trampont AC
  • Macara IG
  • Madhani H
  • Fink GR
  • Ravichandran KS

Journal

Nature cell biology

Publication Data

August 31, 2002

Associated Grants

None

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Caenorhabditis elegans Proteins
  • Carrier Proteins
  • Cell Line
  • Cytoskeletal Proteins
  • Guanine Nucleotide Exchange Factors
  • Guanosine Triphosphate
  • Humans
  • Macromolecular Substances
  • Molecular Sequence Data
  • Phagocytosis
  • Protein Structure, Tertiary
  • Proteins
  • Recombinant Proteins
  • Sequence Homology, Amino Acid
  • rac GTP-Binding Proteins