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Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation.

Cell | Jun 28, 2002

http://www.ncbi.nlm.nih.gov/pubmed/12110186

How membrane receptors initiate signal transduction upon ligand binding is a matter of intense scrutiny. The T cell receptor complex (TCR-CD3) is composed of TCR alpha/beta ligand binding subunits bound to the CD3 subunits responsible for signal transduction. Although it has long been speculated that TCR-CD3 may undergo a conformational change, confirmation is still lacking. We present strong evidence that ligand engagement of TCR-CD3 induces a conformational change that exposes a proline-rich sequence in CD3 epsilon and results in recruitment of the adaptor protein Nck. This occurs earlier than and independently of tyrosine kinase activation. Finally, by interfering with Nck-CD3 epsilon association in vivo, we demonstrate that TCR-CD3 recruitment of Nck is critical for maturation of the immune synapse and for T cell activation.

Pubmed ID: 12110186 RIS Download

Mesh terms: Adaptor Proteins, Signal Transducing | Amino Acid Sequence | Animals | Antigen-Presenting Cells | Antigens, CD3 | Binding Sites | Humans | Jurkat Cells | Ligands | Lymphocyte Activation | Mice | Molecular Sequence Data | Oncogene Proteins | Phosphorylation | Phosphotyrosine | Proline | Protein Binding | Receptors, Antigen, T-Cell | Signal Transduction | T-Lymphocytes | src Homology Domains

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