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The transcriptional regulating protein of 132 kDa (TReP-132) enhances P450scc gene transcription through interaction with steroidogenic factor-1 in human adrenal cells.

The human P450scc gene is regulated by the tissue-specific orphan nuclear receptor, steroidogenic factor-1 (SF-1), which plays a key role in several physiologic processes including steroid synthesis, adrenal and gonadal development, and sexual differentiation. Several studies have demonstrated the interaction of SF-1 with different proteins. However, it is clear that additional factors not yet identified are involved with SF-1 to regulate different target genes. Recently, it was demonstrated that a novel transcriptional regulating protein of 132 kDa (TReP-132) regulates expression of the human P450scc gene. The overexpression of TReP-132 in adrenal cells increases the production of pregnenolone, which is associated with the activation of P450scc gene expression. Considering the colocalization of TReP-132 and SF-1 in steroidogenic tissues such as the adrenal and testis, and the presence of two putative LXXLL motifs in TReP-132 that can potentially interact with SF-1, the relationship between these two factors on the P450scc gene promoter was determined. The coexpression of SF-1 and TReP-132 in adrenal NCI-H295 cells cooperates to increase promoter activity. Pull-down experiments demonstrated the interaction between TReP-132 and SF-1, and this was further confirmed in intact cells by coimmunoprecipitation/Western blot and two-hybrid analyses. Deletions and mutations of the TReP-132 cDNA sequence demonstrate that SF-1 interaction requires the LXXLL motif found at the amino-terminal region of the protein. Also, the "proximal activation domain" and the "AF-2 hexamer" motif of SF-1 are involved in interaction with TReP-132. Consistent with previous studies showing interaction between CBP/p300 and SF-1 or TReP-132, the coexpression of these three proteins results in a synergistic effect on P450scc gene promoter activity. Taken together the results in this study identify a novel function of TReP-132 as a partner in a complex with SF-1 and CBP/p300 to regulate gene transcription involved in steroidogenesis.

Pubmed ID: 12101186


  • Gizard F
  • Lavallee B
  • DeWitte F
  • Teissier E
  • Staels B
  • Hum DW


The Journal of biological chemistry

Publication Data

October 18, 2002

Associated Grants


Mesh Terms

  • Adrenal Gland Neoplasms
  • Adrenal Glands
  • Amino Acid Motifs
  • Animals
  • Blotting, Western
  • Cholesterol Side-Chain Cleavage Enzyme
  • Chromatography, High Pressure Liquid
  • DNA, Complementary
  • DNA-Binding Proteins
  • E1A-Associated p300 Protein
  • Fushi Tarazu Transcription Factors
  • Glutathione Transferase
  • Homeodomain Proteins
  • Humans
  • Luciferases
  • Mice
  • Models, Genetic
  • Nuclear Proteins
  • Plasmids
  • Precipitin Tests
  • Pregnenolone
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Structure, Tertiary
  • Receptors, Cytoplasmic and Nuclear
  • Steroidogenic Factor 1
  • Time Factors
  • Trans-Activators
  • Transcription Factors
  • Transcription, Genetic
  • Transfection
  • Tumor Cells, Cultured