• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression.

The protein kinase NIMA is an indispensable pleiotropic regulator of mitotic progression in Aspergillus. Although several mammalian NIMA-like kinases (Neks) are known, none appears to have the broad importance for mitotic regulation attributed to NIMA. Nercc1 is a new NIMA-like kinase that regulates chromosome alignment and segregation in mitosis. Its NIMA-like catalytic domain is followed by a noncatalytic tail containing seven repeats homologous to those of the Ran GEF, RCC1, a Ser/Thr/Pro-rich segment, and a coiled-coil domain. Nercc1 binds to another NIMA-like kinase, Nek6, and also binds specifically to the Ran GTPase through both its catalytic and its RCC1-like domains, preferring RanGDP in vivo. Nercc1 exists as a homooligomer and can autoactivate in vitro by autophosphorylation. Nercc1 is a cytoplasmic protein that is activated during mitosis and is avidly phosphorylated by active p34(Cdc2). Microinjection of anti-Nercc1 antibodies in prophase results in spindle abnormalities and/or chromosomal misalignment. In Ptk2 cells the outcome is prometaphase arrest or aberrant chromosome segregation and aneuploidy, whereas in CFPAC-1 cells prolonged arrest in prometaphase is the usual response. Nercc1 and its partner Nek6 represent a new signaling pathway that regulates mitotic progression.

Pubmed ID: 12101123

Authors

  • Roig J
  • Mikhailov A
  • Belham C
  • Avruch J

Journal

Genes & development

Publication Data

July 1, 2002

Associated Grants

  • Agency: NIDDK NIH HHS, Id: DK17776

Mesh Terms

  • Amino Acid Sequence
  • Binding Sites
  • CDC2 Protein Kinase
  • Cell Line
  • Cloning, Molecular
  • Humans
  • Immunohistochemistry
  • Mitosis
  • Molecular Sequence Data
  • Protein Binding
  • Protein-Serine-Threonine Kinases
  • Sequence Homology, Amino Acid
  • ran GTP-Binding Protein