Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo.
In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tissues examined, with high levels of GFP expression observed in hematopoetic cells. UBI-GFP/BL6 mice are unique in that B cells, T cells, and dendritic cells have distinct levels of GFP fluorescence. In cell transfer experiments, leukocytes from UBI-GFP/BL6 mice are readily identified by FACS or fluorescence microscopy. We demonstrate that transplanted UBI-GFP/BL6 dendritic cells are easily identified in secondary lymphoid tissues. Direct interactions between individual dendritic cells and multiple naïve CD8+ T cells are observed in lymph nodes within 12 h of cell transfer and require loading of the dendritic cells with the appropriate peptide antigen. Dendritic cells undergo specific morphologic changes following interactions with antigen-specific T cells.
Pubmed ID: 12088410 RIS Download
Adoptive Transfer | Animals | Antigen Presentation | Antigens | CD8-Positive T-Lymphocytes | Cell Communication | Cell Lineage | Cell Movement | Chemotaxis, Leukocyte | Dendritic Cells | Fluorescent Dyes | Gene Expression Regulation | Genes, Reporter | Green Fluorescent Proteins | Luminescent Proteins | Lymph Nodes | Male | Mice | Mice, Inbred C57BL | Mice, Transgenic | Organ Specificity | Promoter Regions, Genetic | Radiation Chimera | Recombinant Fusion Proteins | Spleen | Ubiquitin C