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Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo.

In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tissues examined, with high levels of GFP expression observed in hematopoetic cells. UBI-GFP/BL6 mice are unique in that B cells, T cells, and dendritic cells have distinct levels of GFP fluorescence. In cell transfer experiments, leukocytes from UBI-GFP/BL6 mice are readily identified by FACS or fluorescence microscopy. We demonstrate that transplanted UBI-GFP/BL6 dendritic cells are easily identified in secondary lymphoid tissues. Direct interactions between individual dendritic cells and multiple naïve CD8+ T cells are observed in lymph nodes within 12 h of cell transfer and require loading of the dendritic cells with the appropriate peptide antigen. Dendritic cells undergo specific morphologic changes following interactions with antigen-specific T cells.

Pubmed ID: 12088410

Authors

  • Schaefer BC
  • Schaefer ML
  • Kappler JW
  • Marrack P
  • Kedl RM

Journal

Cellular immunology

Publication Data

December 15, 2001

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI17134
  • Agency: NIAID NIH HHS, Id: AI18785
  • Agency: NIAID NIH HHS, Id: AI22295

Mesh Terms

  • Adoptive Transfer
  • Animals
  • Antigen Presentation
  • Antigens
  • CD8-Positive T-Lymphocytes
  • Cell Communication
  • Cell Lineage
  • Cell Movement
  • Chemotaxis, Leukocyte
  • Dendritic Cells
  • Fluorescent Dyes
  • Gene Expression Regulation
  • Genes, Reporter
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Lymph Nodes
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Organ Specificity
  • Promoter Regions, Genetic
  • Radiation Chimera
  • Recombinant Fusion Proteins
  • Spleen
  • Ubiquitin C