Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo.

Cellular immunology | Dec 15, 2001

In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tissues examined, with high levels of GFP expression observed in hematopoetic cells. UBI-GFP/BL6 mice are unique in that B cells, T cells, and dendritic cells have distinct levels of GFP fluorescence. In cell transfer experiments, leukocytes from UBI-GFP/BL6 mice are readily identified by FACS or fluorescence microscopy. We demonstrate that transplanted UBI-GFP/BL6 dendritic cells are easily identified in secondary lymphoid tissues. Direct interactions between individual dendritic cells and multiple naïve CD8+ T cells are observed in lymph nodes within 12 h of cell transfer and require loading of the dendritic cells with the appropriate peptide antigen. Dendritic cells undergo specific morphologic changes following interactions with antigen-specific T cells.

Pubmed ID: 12088410 RIS Download

Mesh terms: Adoptive Transfer | Animals | Antigen Presentation | Antigens | CD8-Positive T-Lymphocytes | Cell Communication | Cell Lineage | Cell Movement | Chemotaxis, Leukocyte | Dendritic Cells | Fluorescent Dyes | Gene Expression Regulation | Genes, Reporter | Green Fluorescent Proteins | Luminescent Proteins | Lymph Nodes | Male | Mice | Mice, Inbred C57BL | Mice, Transgenic | Organ Specificity | Promoter Regions, Genetic | Radiation Chimera | Recombinant Fusion Proteins | Spleen | Ubiquitin C

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.