We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Induction of ovarian cancer by defined multiple genetic changes in a mouse model system.

Cancer cell | Feb 27, 2002

We have developed a mouse model for ovarian carcinoma by using an avian retroviral gene delivery technique for the introduction of multiple genes into somatic ovarian cells of adult mice. Ovarian cells from transgenic mice engineered to express the gene encoding the avian receptor TVA were efficiently infected in vitro with multiple vectors carrying coding sequences for oncogenes and marker genes. When target cells were derived from TVA transgenic mice deficient for p53, the addition of any two of the oncogenes c-myc, K-ras, and Akt were sufficient to induce ovarian tumor formation when infected cells were injected at subcutaneous, intraperitoneal, or ovarian sites. We demonstrated that the ovarian surface epithelium is the precursor tissue for these ovarian carcinomas, and that introduction of oncogenes causes phenotypic changes in the ovarian surface epithelial cells. The induced ovarian tumors in mice resembled human ovarian carcinomas in their rapid progression and intraperitoneal metastatic spread.

Pubmed ID: 12086888 RIS Download

Mesh terms: Actins | Alkaline Phosphatase | Animals | Avian Proteins | Blotting, Western | Calcium-Binding Proteins | Carcinoma | Cell Division | Cell Transformation, Neoplastic | Disease Models, Animal | Female | Gene Transfer Techniques | Genes, myc | Genes, ras | Genetic Vectors | Humans | Immunoenzyme Techniques | Mice | Mice, Knockout | Mice, Transgenic | Ovarian Neoplasms | Protein-Serine-Threonine Kinases | Proto-Oncogene Proteins | Proto-Oncogene Proteins c-akt | Receptors, Virus | Tumor Suppressor Protein p53

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: R01 CA103924
  • Agency: NCI NIH HHS, Id: R01 CA103924-04
  • Agency: NCI NIH HHS, Id: R01 CA103924-03
  • Agency: NCI NIH HHS, Id: R01 CA103924-02
  • Agency: NCI NIH HHS, Id: R01 CA103924-01

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.