Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

The zinc-finger transcription factor Klf4 is required for terminal differentiation of goblet cells in the colon.

http://www.ncbi.nlm.nih.gov/pubmed/12015290

Klf4 (formerly GKLF) is a zinc-finger transcription factor expressed in the epithelia of the skin, lungs, gastrointestinal tract and several other organs. In vitro studies have suggested that Klf4 plays an important role in cell proliferation and/or differentiation. Mice homozygous for a null mutation in Klf4 die within 15 hours of birth and show selective perturbation of late-stage differentiation structures in the epidermis, but the function of Klf4 in the gastrointestinal tract has not been investigated. To address this issue, we have generated Klf4(-/-) mice by homologous recombination in embryonic stem cells. In this study, we provide the first in vivo evidence that Klf4 is a goblet cell-specific differentiation factor in the colon. Klf4(-/-) mice exhibit normal cell proliferation and cell death rates in the colon on postnatal day 1. However, Klf4(-/-) mice demonstrate a 90% decrease in the number of goblet cells in the colon, show abnormal expression of the goblet cell-specific marker Muc2 by in situ hybridization, have abnormal staining of the colonic epithelium with Alcian Blue for acidic mucins, and lack normal goblet cell morphology by ultrastructural analysis. All other epithelial cell types are present in the colon of Klf4(-/-) mice. In summary, Klf4 plays a crucial role in colonic epithelial cell differentiation in vivo.

Pubmed ID: 12015290 RIS Download

Mesh terms: Animals | Base Sequence | Cell Death | Cell Differentiation | Cell Division | Colon | DNA-Binding Proteins | Exons | Genotype | Goblet Cells | Intestinal Mucosa | Kruppel-Like Transcription Factors | Mice | Mice, Knockout | Polymerase Chain Reaction | Restriction Mapping | Transcription Factors | Zinc Fingers

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIDDK NIH HHS, Id: K08 DK02809-01
  • Agency: NIDDK NIH HHS, Id: P30 DK50306
  • Agency: NCI NIH HHS, Id: R01 CA084197
  • Agency: NCI NIH HHS, Id: R01 CA84197
  • Agency: NIDDK NIH HHS, Id: R01 DK052230
  • Agency: NIDDK NIH HHS, Id: R01 DK052230-04
  • Agency: NIDDK NIH HHS, Id: R01 DK52230
  • Agency: NIDDK NIH HHS, Id: R01 DK53839

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.