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A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells.

Science (New York, N.Y.) | May 10, 2002

http://www.ncbi.nlm.nih.gov/pubmed/12004135

E2F-6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. To better elucidate the molecular mechanism of repression by E2F-6, we have purified the factor from cultured cells. E2F-6 is found in a multimeric protein complex that contains Mga and Max, and thus the complex can bind not only to the E2F-binding site but also to Myc- and Brachyury-binding sites. Moreover, the complex contains chromatin modifiers such as a novel histone methyltransferase that modifies lysine 9 of histone H3, HP1gamma, and Polycomb group (PcG) proteins. The E2F-6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells. These data suggest that these chromatin modifiers contribute to silencing of E2F- and Myc-responsive genes in quiescent cells.

Pubmed ID: 12004135 RIS Download

Mesh terms: Amino Acid Sequence | Binding Sites | Cell Cycle Proteins | Chromatin | Chromosomal Proteins, Non-Histone | DNA-Binding Proteins | Dimerization | E2F Transcription Factors | E2F6 Transcription Factor | G0 Phase | G1 Phase | Gene Silencing | HeLa Cells | Histone-Lysine N-Methyltransferase | Histones | Humans | Mass Spectrometry | Methylation | Methyltransferases | Molecular Sequence Data | Phosphoproteins | Promoter Regions, Genetic | Protein Footprinting | Protein Methyltransferases | Proteins | Proto-Oncogene Proteins c-myc | Recombinant Proteins | Retinoblastoma-Like Protein p130 | Transcription Factors | Transcription, Genetic | Two-Hybrid System Techniques

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