• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells.

E2F-6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. To better elucidate the molecular mechanism of repression by E2F-6, we have purified the factor from cultured cells. E2F-6 is found in a multimeric protein complex that contains Mga and Max, and thus the complex can bind not only to the E2F-binding site but also to Myc- and Brachyury-binding sites. Moreover, the complex contains chromatin modifiers such as a novel histone methyltransferase that modifies lysine 9 of histone H3, HP1gamma, and Polycomb group (PcG) proteins. The E2F-6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells. These data suggest that these chromatin modifiers contribute to silencing of E2F- and Myc-responsive genes in quiescent cells.

Pubmed ID: 12004135


  • Ogawa H
  • Ishiguro K
  • Gaubatz S
  • Livingston DM
  • Nakatani Y


Science (New York, N.Y.)

Publication Data

May 10, 2002

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Binding Sites
  • Cell Cycle Proteins
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • Dimerization
  • E2F Transcription Factors
  • E2F6 Transcription Factor
  • G0 Phase
  • G1 Phase
  • Gene Silencing
  • HeLa Cells
  • Histone-Lysine N-Methyltransferase
  • Histones
  • Humans
  • Mass Spectrometry
  • Methylation
  • Methyltransferases
  • Molecular Sequence Data
  • Phosphoproteins
  • Promoter Regions, Genetic
  • Protein Footprinting
  • Protein Methyltransferases
  • Proteins
  • Proto-Oncogene Proteins c-myc
  • Recombinant Proteins
  • Retinoblastoma-Like Protein p130
  • Transcription Factors
  • Transcription, Genetic
  • Two-Hybrid System Techniques