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Cyclin L is an RS domain protein involved in pre-mRNA splicing.

We report the cDNA cloning and functional characterization of human cyclin L, a novel cyclin related to the C-type cyclins that are involved in regulation of RNA polymerase II (pol II) transcription. Cyclin L also contains a COOH-terminal dipeptide repeat of alternating arginines and serines, a hallmark of the SR family of splicing factors. We show that recombinant cyclin L interacts with p110 PITSLRE kinase, and that cyclin L antibody co-immunoprecipitates a kinase activity from HeLa nuclear extracts that phosphorylates the carboxyl-terminal domain (CTD) of pol II and splicing factor SC35, and is inhibited by the cdk inhibitor p21. Cyclin L antibody inhibits the second step of RNA splicing in vitro, and recombinant cyclin L protein stimulates splicing under suboptimal conditions. Significantly, the IC(50) for splicing inhibition by p21 is similar to the IC(50) for inhibition of the cyclin L-associated kinase activity. Cyclin L and its associated kinase are thus new members of the pre-mRNA processing machinery.

Pubmed ID: 11980906


  • Dickinson LA
  • Edgar AJ
  • Ehley J
  • Gottesfeld JM


The Journal of biological chemistry

Publication Data

July 12, 2002

Associated Grants


Mesh Terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cyclins
  • DNA Primers
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Precipitin Tests
  • RNA Precursors
  • RNA, Messenger
  • Recombinant Proteins
  • Sequence Homology, Amino Acid