• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Cyclin L is an RS domain protein involved in pre-mRNA splicing.

We report the cDNA cloning and functional characterization of human cyclin L, a novel cyclin related to the C-type cyclins that are involved in regulation of RNA polymerase II (pol II) transcription. Cyclin L also contains a COOH-terminal dipeptide repeat of alternating arginines and serines, a hallmark of the SR family of splicing factors. We show that recombinant cyclin L interacts with p110 PITSLRE kinase, and that cyclin L antibody co-immunoprecipitates a kinase activity from HeLa nuclear extracts that phosphorylates the carboxyl-terminal domain (CTD) of pol II and splicing factor SC35, and is inhibited by the cdk inhibitor p21. Cyclin L antibody inhibits the second step of RNA splicing in vitro, and recombinant cyclin L protein stimulates splicing under suboptimal conditions. Significantly, the IC(50) for splicing inhibition by p21 is similar to the IC(50) for inhibition of the cyclin L-associated kinase activity. Cyclin L and its associated kinase are thus new members of the pre-mRNA processing machinery.

Pubmed ID: 11980906


  • Dickinson LA
  • Edgar AJ
  • Ehley J
  • Gottesfeld JM


The Journal of biological chemistry

Publication Data

July 12, 2002

Associated Grants


Mesh Terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cyclins
  • DNA Primers
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Precipitin Tests
  • RNA Precursors
  • RNA, Messenger
  • Recombinant Proteins
  • Sequence Homology, Amino Acid