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Identification of essential regions in the cytoplasmic tail of interleukin-1 receptor accessory protein critical for interleukin-1 signaling.

Interleukin (IL)-1 plays an important role in inflammation and regulation of immune responses. The activated IL-1 receptor complex, which consists of the IL-1 receptor type I and the IL-1 receptor accessory protein (IL-1RAcP), generates multiple cellular responses including NF-kappaB activation, IL-2 secretion, and IL-2 promoter activation. Reconstitution experiments in EL4D6/76 cells lacking IL-1RAcP expression and IL-1 responsiveness were used to analyze structure-function relationships of the IL-1RAcP cytoplasmic tail. Mutating a potential tyrosine kinase phosphorylation motif and various conserved amino acid (aa) residues had no effect on IL-1 responsiveness. Truncation analyses revealed that box 3 of the TIR domain was required for NF-kappaB activation, IL-2 production, and c-Jun N-terminal kinase (JNK) activation, whereas IL-2 promoter activation was only partially inhibited. Surprisingly, deletion of aa 527-534 resulted in almost complete loss of all IL-1 responsiveness. Replacement of these aa with alanyl residues did not reconstitute NF-kappaB activation, IL-2 production, or JNK activation but partly restored IL-2 promoter activation. Immunoprecipitation data revealed a strong correlation between MyD88 binding with NF-kappaB activation and IL-2 production but not with IL-2 promoter activation. Taken together, our data indicate that box 3 of IL-1RAcP is critical for IL-1-dependent NF-kappaB activation and stabilization of IL-2 mRNA via JNK, whereas aa 527-534 largely contribute to IL-2 promoter activation.

Pubmed ID: 11880380 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Blotting, Western | Cell Line | Cytoplasm | Enzyme Activation | Enzyme-Linked Immunosorbent Assay | Gene Deletion | Genes, Reporter | Humans | Interleukin-1 | Interleukin-1 Receptor Accessory Protein | Interleukin-2 | JNK Mitogen-Activated Protein Kinases | Luciferases | Mice | Mitogen-Activated Protein Kinases | Models, Biological | Molecular Sequence Data | Mutation | NF-kappa B | Plasmids | Precipitin Tests | Promoter Regions, Genetic | Protein Binding | Protein Structure, Tertiary | Proteins | Signal Transduction | Structure-Activity Relationship | Time Factors | Transfection

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