Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Protein kinase C delta regulates function of the DF3/MUC1 carcinoma antigen in beta-catenin signaling.

http://www.ncbi.nlm.nih.gov/pubmed/11877440

The DF3/MUC1 mucin-like glycoprotein is aberrantly overexpressed in most human carcinomas. The MUC1 cytoplasmic domain interacts directly with beta-catenin, a component of the adherens junction of mammalian epithelial cells. The present results demonstrate that MUC1 associates with protein kinase Cdelta (PKCdelta). A TDR sequence adjacent to the beta-catenin binding motif in the MUC1 cytoplasmic domain functions as a site for PKCdelta phosphorylation. We show that phosphorylation of MUC1 by PKCdelta increases binding of MUC1 and beta-catenin in vitro and in vivo. The functional significance of the MUC1-PKCdelta interaction is further supported by the demonstration that mutation of the PKCdelta phosphorylation site abrogates MUC1-mediated decreases in binding of beta-catenin to E-cadherin. We also show that the stimulatory effects of MUC1 on anchorage-independent growth are abrogated by mutation of the PKCdelta phosphorylation site. These findings support a novel role for PKCdelta in regulating the interaction between MUC1 and the beta-catenin signaling pathway.

Pubmed ID: 11877440 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Antigens, Neoplasm | Base Sequence | Cadherins | Cell Adhesion | Cytoskeletal Proteins | Electrophoresis, Polyacrylamide Gel | Female | Humans | Isoenzymes | Mice | Molecular Sequence Data | Phosphorylation | Protein Binding | Protein Kinase C | Protein Kinase C-delta | Trans-Activators | Tumor Cells, Cultured | beta Catenin

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: CA87421

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.