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Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform.

The molecular mechanisms underlying early/recycling endosomes-to-TGN transport are still not understood. We identified interactions between the TGN-localized putative t-SNAREs syntaxin 6, syntaxin 16, and Vti1a, and two early/recycling endosomal v-SNAREs, VAMP3/cellubrevin, and VAMP4. Using a novel permeabilized cell system, these proteins were functionally implicated in the post-Golgi retrograde transport step. The function of Rab6a' was also required, whereas its closely related isoform, Rab6a, has previously been implicated in Golgi-to-endoplasmic reticulum transport. Thus, our study shows that membrane exchange between the early endocytic and the biosynthetic/secretory pathways involves specific components of the Rab and SNARE machinery, and suggests that retrograde transport between early/recycling endosomes and the endoplasmic reticulum is critically dependent on the sequential action of two members of the Rab6 subfamily.

Pubmed ID: 11839770


  • Mallard F
  • Tang BL
  • Galli T
  • Tenza D
  • Saint-Pol A
  • Yue X
  • Antony C
  • Hong W
  • Goud B
  • Johannes L


The Journal of cell biology

Publication Data

February 18, 2002

Associated Grants


Mesh Terms

  • Animals
  • Biological Transport, Active
  • CHO Cells
  • Carrier Proteins
  • Cricetinae
  • Endosomes
  • HeLa Cells
  • Humans
  • Membrane Proteins
  • Protein Isoforms
  • Qa-SNARE Proteins
  • Qb-SNARE Proteins
  • SNARE Proteins
  • Shiga Toxins
  • Syntaxin 16
  • Vesicle-Associated Membrane Protein 3
  • Vesicular Transport Proteins
  • rab GTP-Binding Proteins
  • trans-Golgi Network