Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin.

Cell | Jan 25, 2002

http://www.ncbi.nlm.nih.gov/pubmed/11832213

The ADF (actin-depolymerizing factor)/cofilin family is a stimulus-responsive mediator of actin dynamics. In contrast to the mechanisms of inactivation of ADF/cofilin by kinases such as LIM-kinase 1 (LIMK1), much less is known about its reactivation through dephosphorylation. Here we report Slingshot (SSH), a family of phosphatases that have the property of F actin binding. In Drosophila, loss of ssh function dramatically increased levels of both F actin and phospho-cofilin (P cofilin) and disorganized epidermal cell morphogenesis. In mammalian cells, human SSH homologs (hSSHs) suppressed LIMK1-induced actin reorganization. Furthermore, SSH and the hSSHs dephosphorylated P cofilin in cultured cells and in cell-free assays. Our results strongly suggest that the SSH family plays a pivotal role in actin dynamics by reactivating ADF/cofilin in vivo.

Pubmed ID: 11832213 RIS Download

Mesh terms: Actin Depolymerizing Factors | Actins | Amino Acid Sequence | Animals | Cell Line | Cell-Free System | Destrin | Drosophila | Drosophila Proteins | Embryonic Structures | Homeodomain Proteins | Humans | Immunohistochemistry | LIM-Homeodomain Proteins | Microfilament Proteins | Molecular Sequence Data | Phosphoprotein Phosphatases | Phosphoric Monoester Hydrolases | Phosphorylation | Protein Binding | Protein-Serine-Threonine Kinases | Sequence Alignment | Transcription Factors | Zyxin

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.