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A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3'-end processing.

Genes & development | Jan 1, 2002

http://www.ncbi.nlm.nih.gov/pubmed/11782445

The stem-loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells. SLBP binds histone pre-mRNAs and facilitates 3'-end processing by promoting stable association of U7 snRNP with the pre-mRNA. To identify other factors involved in histone pre-mRNA processing, we used a modified yeast two-hybrid assay in which SLBP and its RNA target were coexpressed as bait. A novel zinc finger protein, hZFP100, which interacts with the SLBP/RNA complex but not with free SLBP, was cloned. The interaction requires regions of SLBP that are important for histone pre-mRNA processing. Antibodies to hZFP100 precipitate U7 snRNA, and expression of hZFP100 in Xenopus oocytes stimulates processing of histone pre-mRNA, showing that hZFP100 is a component of the processing machinery.

Pubmed ID: 11782445 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Base Sequence | Cloning, Molecular | DNA-Binding Proteins | HeLa Cells | Histones | Humans | Molecular Sequence Data | Nuclear Proteins | Peptide Mapping | RNA Precursors | RNA Processing, Post-Transcriptional | RNA-Binding Proteins | Ribonucleoprotein, U7 Small Nuclear | Xenopus | Xenopus Proteins | Zinc Fingers | mRNA Cleavage and Polyadenylation Factors

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