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A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3'-end processing.

The stem-loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells. SLBP binds histone pre-mRNAs and facilitates 3'-end processing by promoting stable association of U7 snRNP with the pre-mRNA. To identify other factors involved in histone pre-mRNA processing, we used a modified yeast two-hybrid assay in which SLBP and its RNA target were coexpressed as bait. A novel zinc finger protein, hZFP100, which interacts with the SLBP/RNA complex but not with free SLBP, was cloned. The interaction requires regions of SLBP that are important for histone pre-mRNA processing. Antibodies to hZFP100 precipitate U7 snRNA, and expression of hZFP100 in Xenopus oocytes stimulates processing of histone pre-mRNA, showing that hZFP100 is a component of the processing machinery.

Pubmed ID: 11782445

Authors

  • Dominski Z
  • Erkmann JA
  • Yang X
  • S├ánchez R
  • Marzluff WF

Journal

Genes & development

Publication Data

January 1, 2002

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM29832

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA-Binding Proteins
  • HeLa Cells
  • Histones
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins
  • Peptide Mapping
  • RNA Precursors
  • RNA Processing, Post-Transcriptional
  • RNA-Binding Proteins
  • Ribonucleoprotein, U7 Small Nuclear
  • Xenopus
  • Xenopus Proteins
  • Zinc Fingers
  • mRNA Cleavage and Polyadenylation Factors