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Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding.

GABARAP recognizes and binds the gamma2 subunit of the GABA(A) receptor, interacts with microtubules and the N-ethyl maleimide sensitive factor, and is proposed to function in GABA(A) receptor trafficking and postsynaptic localization. We have determined the crystal structure of human GABARAP at 1.6 A resolution. The structure comprises an N-terminal helical subdomain and a ubiquitin-like C-terminal domain. Structure-based mutational analysis demonstrates that the N-terminal subdomain is responsible for tubulin binding while the C-terminal domain contains the binding site for the GABA(A). A second GABARAP crystal form was determined at 1.9 A resolution and documents that GABARAP can self-associate in a head-to-tail manner. The structural details of this oligomerization reveal how GABARAP can both promote tubulin polymerization and facilitate GABA(A) receptor clustering.

Pubmed ID: 11779480

Authors

  • Coyle JE
  • Qamar S
  • Rajashankar KR
  • Nikolov DB

Journal

Neuron

Publication Data

January 3, 2002

Associated Grants

None

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Binding Sites
  • Humans
  • Microtubule-Associated Proteins
  • Microtubules
  • Molecular Sequence Data
  • Neural Inhibition
  • Polymers
  • Protein Structure, Tertiary
  • Protein Transport
  • Receptors, GABA-A
  • Sequence Homology, Amino Acid
  • Synaptic Membranes
  • Synaptic Transmission
  • Tubulin