Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Wallerian degeneration of injured axons and synapses is delayed by a Ube4b/Nmnat chimeric gene.

Nature neuroscience | Dec 26, 2001

Axons and their synapses distal to an injury undergo rapid Wallerian degeneration, but axons in the C57BL/WldS mouse are protected. The degenerative and protective mechanisms are unknown. We identified the protective gene, which encodes an N-terminal fragment of ubiquitination factor E4B (Ube4b) fused to nicotinamide mononucleotide adenylyltransferase (Nmnat), and showed that it confers a dose-dependent block of Wallerian degeneration. Transected distal axons survived for two weeks, and neuromuscular junctions were also protected. Surprisingly, the Wld protein was located predominantly in the nucleus, indicating an indirect protective mechanism. Nmnat enzyme activity, but not NAD+ content, was increased fourfold in WldS tissues. Thus, axon protection is likely to be mediated by altered ubiquitination or pyridine nucleotide metabolism.

Pubmed ID: 11770485 RIS Download

Mesh terms: Action Potentials | Animals | Axons | Base Sequence | Cell Nucleus | Cell Survival | Fungal Proteins | Immunohistochemistry | Mice | Mice, Mutant Strains | Mice, Transgenic | Microscopy, Electron | Molecular Sequence Data | Motor Neurons | Muscle, Skeletal | Mutation | Nerve Tissue Proteins | Nervous System | Neuromuscular Junction | Nicotinamide-Nucleotide Adenylyltransferase | Recombinant Fusion Proteins | Saccharomyces cerevisiae Proteins | Sciatic Nerve | Synaptic Transmission | Synaptic Vesicles | Trauma, Nervous System | Ubiquitin-Conjugating Enzymes | Wallerian Degeneration

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.