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Cell surface glycoprotein PZR is a major mediator of concanavalin A-induced cell signaling.

PZR is an immunoglobulin superfamily cell surface protein containing a pair of immunoreceptor tyrosine-based inhibitory motifs. As a glycoprotein, PZR displays a strong association with concanavalin A (ConA), a member of the plant lectin family. Treatment of several cell lines with ConA caused tyrosine phosphorylation of a major cellular protein. Immunoblotting and immunoprecipitation studies indicated that this protein corresponded to PZR. Tyrosine phosphorylation of PZR was accompanied by recruitment of SHP-2 and was inhibited by PP1, a selective inhibitor of the Src family tyrosine kinases. Furthermore, c-Src was constitutively associated with PZR and was activated upon treatment of cells with ConA. Moreover, tyrosine phosphorylation of PZR was markedly enhanced in v-Src-transformed NIH-3T3 cells and was predominant in Escherichia coli cells co-expressing c-Src. Expression of an intracellular domain-truncated form of PZR in HT-1080 cells affected cell morphology and had a dominant negative effect on ConA-induced tyrosine phosphorylation of PZR, activation of c-Src, and agglutination of the cells. Together, the data indicate that PZR is a major receptor of ConA and has an important role in cell signaling via c-Src. Considering the various biological activities of ConA, the study of PZR may have major therapeutic implications.

Pubmed ID: 11751924

Authors

  • Zhao R
  • Guerrah A
  • Tang H
  • Zhao ZJ

Journal

The Journal of biological chemistry

Publication Data

March 8, 2002

Associated Grants

  • Agency: NCI NIH HHS, Id: CA68485
  • Agency: NCI NIH HHS, Id: CA75218
  • Agency: NIDDK NIH HHS, Id: DK15555
  • Agency: NHLBI NIH HHS, Id: HL57393
  • Agency: NHLBI NIH HHS, Id: HL69806
  • Agency: NIDDK NIH HHS, Id: T32-DK07186

Mesh Terms

  • 3T3 Cells
  • Amino Acid Motifs
  • Animals
  • Blotting, Western
  • Cell Line
  • Concanavalin A
  • Dose-Response Relationship, Drug
  • Escherichia coli
  • HeLa Cells
  • Humans
  • Immunoblotting
  • Mice
  • Oncogene Protein pp60(v-src)
  • Phosphorylation
  • Precipitin Tests
  • Protein Binding
  • Protein Structure, Tertiary
  • Signal Transduction
  • Time Factors
  • Tyrosine