• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

ATR and ATRIP: partners in checkpoint signaling.

The checkpoint kinases ATM (ataxia telangiectasia mutated) and ATR (ATM and Rad3 related) transduce genomic stress signals to halt cell cycle progression and promote DNA repair. We report the identification of an ATR-interacting protein (ATRIP) that is phosphorylated by ATR, regulates ATR expression, and is an essential component of the DNA damage checkpoint pathway. ATR and ATRIP both localize to intranuclear foci after DNA damage or inhibition of replication. Deletion of ATR mediated by the Cre recombinase caused the loss of ATR and ATRIP expression, loss of DNA damage checkpoint responses, and cell death. Therefore, ATR is essential for the viability of human somatic cells. Small interfering RNA directed against ATRIP caused the loss of both ATRIP and ATR expression and the loss of checkpoint responses to DNA damage. Thus, ATRIP and ATR are mutually dependent partners in cell cycle checkpoint signaling pathways.

Pubmed ID: 11721054

Authors

  • Cortez D
  • Guntuku S
  • Qin J
  • Elledge SJ

Journal

Science (New York, N.Y.)

Publication Data

November 23, 2001

Associated Grants

None

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Ataxia Telangiectasia Mutated Proteins
  • Cell Cycle
  • Cell Cycle Proteins
  • Cell Death
  • Cell Line
  • Cell Survival
  • Conserved Sequence
  • DNA Damage
  • DNA-Binding Proteins
  • Exodeoxyribonucleases
  • Exons
  • Gene Deletion
  • Genes, Essential
  • HeLa Cells
  • Humans
  • Integrases
  • Molecular Sequence Data
  • Molecular Weight
  • Phosphoproteins
  • Phosphorylation
  • Precipitin Tests
  • Protein Binding
  • Protein-Serine-Threonine Kinases
  • Sequence Alignment
  • Signal Transduction
  • Viral Proteins