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Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps.

Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p contains a putative GTP-binding site, which is essential in vivo. Kinetic and steady-state measurements of the levels of pre-rRNAs in Nog2p-depleted cells showed a defect in 5.8S and 25S maturation and a concomitant increase in the levels of both 27SB(S) and 7S(S) precursors. We found Nog2p physically associated with large pre-60S complexes highly enriched in the 27SB and 7S rRNA precursors. These complexes contained, besides a subset of ribosomal proteins, at least two additional factors, Nog1p, another putative GTP-binding protein, and Rlp24p (Ylr009wp), which belongs to the Rpl24e family of archaeal and eukaryotic ribosomal proteins. In the absence of Nog2p, the pre-60S ribosomal complexes left the nucleolus, but were retained in the nucleoplasm. These results suggest that transient, possibly GTP-dependent association of Nog2p with the pre-ribosomes might trigger late rRNA maturation steps in ribosomal large subunit biogenesis.

Pubmed ID: 11707418

Authors

  • Saveanu C
  • Bienvenu D
  • Namane A
  • Gleizes PE
  • Gas N
  • Jacquier A
  • Fromont-Racine M

Journal

The EMBO journal

Publication Data

November 15, 2001

Associated Grants

None

Mesh Terms

  • Active Transport, Cell Nucleus
  • Alternative Splicing
  • Amino Acid Sequence
  • Binding Sites
  • Blotting, Northern
  • Cell Nucleolus
  • Cell Nucleus
  • Cytoplasm
  • DNA, Complementary
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • Genotype
  • Glucose
  • Green Fluorescent Proteins
  • Humans
  • In Situ Hybridization
  • Kinetics
  • Luminescent Proteins
  • Mass Spectrometry
  • Microscopy, Electron
  • Microscopy, Fluorescence
  • Models, Genetic
  • Molecular Sequence Data
  • Plasmids
  • Polyribosomes
  • Promoter Regions, Genetic
  • Protein Binding
  • RNA
  • RNA, Messenger
  • RNA, Ribosomal
  • Ribosomes
  • Sequence Homology, Amino Acid
  • Time Factors