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G beta association and effector interaction selectivities of the divergent G gamma subunit G gamma(13).

G gamma(13) is a divergent member of the G gamma subunit family considered to be a component of the gustducin G-protein heterotrimer involved in bitter and sweet taste reception in taste bud cells. G gamma(13) contains a C-terminal asparagine-proline-tryptophan (NPW) tripeptide, a hallmark of RGS protein G gamma-like (GGL) domains which dimerize exclusively with G beta(5) subunits. In this study, we investigated the functional range of G gamma(13) assembly with G beta subunits using multiple assays of G beta association and G beta gamma effector modulation. G gamma(13) was observed to associate with all five G beta subunits (G beta(1-5)) upon co-translation in vitro, as well as function with all five G beta subunits in the modulation of Kir3.1/3.4 (GIRK1/4) potassium and N-type (alpha(1B)) calcium channels. Multiple G beta/G gamma(13) pairings were also functional in cellular assays of phospholipase C (PLC) beta 2 activation and inhibition of G alpha(q)-stimulated PLC beta 1 activity. However, upon cellular co-expression of G gamma(13) with different G beta subunits, only G beta(1)/G gamma(13), G beta(3)/G gamma(13), and G beta(4)/G gamma(13) pairings were found to form stable dimers detectable by co-immunoprecipitation under high-detergent cell lysis conditions. Collectively, these data indicate that G gamma(13) forms functional G beta gamma dimers with a range of G beta subunits. Coupled with our detection of G gamma(13) mRNA in mouse and human brain and retina, these results imply that this divergent G gamma subunit can act in signal transduction pathways other than that dedicated to taste reception in sensory lingual tissue.

Pubmed ID: 11675383


  • Blake BL
  • Wing MR
  • Zhou JY
  • Lei Q
  • Hillmann JR
  • Behe CI
  • Morris RA
  • Harden TK
  • Bayliss DA
  • Miller RJ
  • Siderovski DP


The Journal of biological chemistry

Publication Data

December 28, 2001

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM29536
  • Agency: NIGMS NIH HHS, Id: GM62338
  • Agency: NIMH NIH HHS, Id: MH001896
  • Agency: NINDS NIH HHS, Id: NS33826
  • Agency: NINDS NIH HHS, Id: NS39553
  • Agency: NINDS NIH HHS, Id: R01 NS039553

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Calcium Channels, N-Type
  • Cell Line
  • Dimerization
  • Enzyme Activation
  • Green Fluorescent Proteins
  • Heterotrimeric GTP-Binding Proteins
  • Humans
  • Indicators and Reagents
  • Isoenzymes
  • Luminescent Proteins
  • Molecular Sequence Data
  • Patch-Clamp Techniques
  • Phospholipase C beta
  • Potassium Channels
  • Protein Isoforms
  • Protein Subunits
  • Sequence Alignment
  • Tissue Distribution
  • Type C Phospholipases