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Sulfation of L-selectin ligands by an HEV-restricted sulfotransferase regulates lymphocyte homing to lymph nodes.

Lymphocytes home to lymph nodes, using L-selectin to bind specific ligands on high endothelial venules (HEV). In vitro studies implicate GlcNAc-6-sulfate as an essential posttranslational modification for ligand activity. Here, we show that genetic deletion of HEC-GlcNAc6ST, a sulfotransferase that is highly restricted to HEV, results in the loss of the binding of recombinant L-selectin to the luminal aspect of HEV, elimination of lymphocyte binding in vitro, and markedly reduced in vivo homing. Reactivity with MECA 79, an adhesion-blocking mAb that stains HEV in lymph nodes and vessels in chronic inflammatory sites, is also lost from the luminal aspects of HEV. These results establish a critical role for HEC-GlcNAc6ST in lymphocyte trafficking and suggest it as an important therapeutic target.

Pubmed ID: 11520459


  • Hemmerich S
  • Bistrup A
  • Singer MS
  • van Zante A
  • Lee JK
  • Tsay D
  • Peters M
  • Carminati JL
  • Brennan TJ
  • Carver-Moore K
  • Leviten M
  • Fuentes ME
  • Ruddle NH
  • Rosen SD



Publication Data

August 24, 2001

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI 44453
  • Agency: NCI NIH HHS, Id: CA16885
  • Agency: NIGMS NIH HHS, Id: GM57411

Mesh Terms

  • Animals
  • Cell Adhesion
  • Chemotaxis, Leukocyte
  • L-Selectin
  • Lectins
  • Ligands
  • Lymph Nodes
  • Lymphatic System
  • Lymphocytes
  • Mice
  • Mice, Mutant Strains
  • Sulfotransferases