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Identification and characterization of a family of Rab11-interacting proteins.

Rab11a is a small GTP-binding protein enriched in the pericentriolar plasma membrane recycling systems. We hypothesized that Rab11a-binding proteins exist as downstream effectors of its action. Here we define a family of four Rab11-interacting proteins: Rab11-Family Interacting Protein 1 (Rab11-FIP1), Rab11-Family Interacting Protein 2 (Rab11-FIP2), Rab11-Family Interacting Protein 3 (Rab11-FIP3), and pp75/Rip11. All four interacting proteins associated with wild type Rab11a and dominant active Rab11a (Rab11aS20V) as well as Rab11b and Rab25. Rab11-FIP2 also interacted with dominant negative Rab11a (Rab11aS25N) and the tail of myosin Vb. The binding of Rab11-FIP1, Rab11-FIP2, and Rab11-FIP3 to Rab11a was dependent upon a conserved carboxyl-terminal amphipathic alpha-helix. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 colocalized with Rab11a in plasma membrane recycling systems in both non-polarized HeLa cells and polarized Madin-Darby canine kidney cells. GFP-Rab11-FIP3 also colocalized with Rab11a in HeLa cells. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 also coenriched with Rab11a and H(+)K(+)-ATPase on parietal cell tubulovesicles, and Rab11-FIP1 and Rab11-FIP2 translocated with Rab11a and the H(+)K(+)-ATPase upon stimulating parietal cells with histamine. The results suggest that the function of Rab11a in plasma membrane recycling systems is dependent upon a compendium of protein effectors.

Pubmed ID: 11495908

Authors

  • Hales CM
  • Griner R
  • Hobdy-Henderson KC
  • Dorn MC
  • Hardy D
  • Kumar R
  • Navarre J
  • Chan EK
  • Lapierre LA
  • Goldenring JR

Journal

The Journal of biological chemistry

Publication Data

October 19, 2001

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI47859
  • Agency: NIDDK NIH HHS, Id: DK43405
  • Agency: NIDDK NIH HHS, Id: DK48370

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Antineoplastic Agents
  • Antineoplastic Agents, Phytogenic
  • Base Sequence
  • Blotting, Western
  • Cell Line
  • Cell Membrane
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary
  • Dogs
  • Expressed Sequence Tags
  • Gastric Mucosa
  • Gene Deletion
  • Gene Library
  • Genes, Dominant
  • HeLa Cells
  • Histamine
  • Humans
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Nocodazole
  • Paclitaxel
  • Protein Binding
  • Rabbits
  • Recombinant Proteins
  • Sequence Homology, Amino Acid
  • Two-Hybrid System Techniques
  • rab GTP-Binding Proteins