Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Multiple phosphorylated isoforms of NRL are expressed in rod photoreceptors.

http://www.ncbi.nlm.nih.gov/pubmed/11477108

NRL, a bZIP transcription factor of the Maf subfamily, interacts with the homeodomain protein CRX and synergistically regulates rhodopsin expression. Here we report that six isoforms of NRL (29-35 kDa) are generated by phosphorylation and expressed specifically in the mammalian retina. The anti-NRL antibody also cross-reacts with a cytosolic 45-kDa protein, which is detected in neuronal tissues but is not encoded by the NRL gene. In both human retinal cell cultures and sections of fetal and adult human retina, NRL is present in the nuclei of developing and mature rods but not cones. We propose that NRL regulates rod photoreceptor-specific gene expression and is involved in rod differentiation.

Pubmed ID: 11477108 RIS Download

Mesh terms: Alkaline Phosphatase | Animals | Basic-Leucine Zipper Transcription Factors | COS Cells | Cell Differentiation | Cell Nucleus | Cells, Cultured | DNA-Binding Proteins | Electrophoresis, Polyacrylamide Gel | Eye Proteins | Humans | Immunoblotting | Immunohistochemistry | Mice | Mice, Knockout | Phosphoric Monoester Hydrolases | Phosphorylation | Protein Isoforms | Recombinant Proteins | Retina | Retinal Rod Photoreceptor Cells | Rhodopsin | Time Factors | Transfection

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NEI NIH HHS, Id: EY01730
  • Agency: NEI NIH HHS, Id: EY04536
  • Agency: NEI NIH HHS, Id: EY07003
  • Agency: NEI NIH HHS, Id: EY11115

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.