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dpl-1 DP and efl-1 E2F act with lin-35 Rb to antagonize Ras signaling in C. elegans vulval development.

The synthetic multivulva (synMuv) genes define two functionally redundant pathways that antagonize RTK/Ras signaling during Caenorhabditis elegans vulval induction. The synMuv gene lin-35 encodes a protein similar to the mammalian tumor suppressor pRB and has been proposed to act as a transcriptional repressor. Studies using mammalian cells have shown that pRB can prevent cell cycle progression by inhibiting DP/E2F-mediated transcriptional activation. We identified C. elegans genes that encode proteins similar to DP or E2F. Loss-of-function mutations in two of these genes, dpl-1 DP and efl-1 E2F, caused the same vulval abnormalities as do lin-35 Rb loss-of-function mutations. We propose that rather than being inhibited by lin-35 Rb, dpl-1 DP and efl-1 E2F act with lin-35 Rb in transcriptional repression to antagonize RTK/Ras signaling during vulval development.

Pubmed ID: 11463372

Authors

  • Ceol CJ
  • Horvitz HR

Journal

Molecular cell

Publication Data

March 15, 2001

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM24663

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins
  • Cell Cycle Proteins
  • Cell Division
  • Cell Lineage
  • Cell Nucleus
  • Cloning, Molecular
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • Female
  • Gene Deletion
  • Gene Expression Regulation, Developmental
  • Genes, Helminth
  • Helminth Proteins
  • Membrane Glycoproteins
  • Molecular Sequence Data
  • Neurons
  • Nuclear Proteins
  • Phenotype
  • Protein Binding
  • Repressor Proteins
  • Signal Transduction
  • Transcription Factors
  • Vulva
  • ras Proteins