RNAi is a gene-silencing phenomenon triggered by double-stranded (ds) RNA and involves the generation of 21 to 26 nt RNA segments that guide mRNA destruction. In Caenorhabditis elegans, lin-4 and let-7 encode small temporal RNAs (stRNAs) of 22 nt that regulate stage-specific development. Here we show that inactivation of genes related to RNAi pathway genes, a homolog of Drosophila Dicer (dcr-1), and two homologs of rde-1 (alg-1 and alg-2), cause heterochronic phenotypes similar to lin-4 and let-7 mutations. Further we show that dcr-1, alg-1, and alg-2 are necessary for the maturation and activity of the lin-4 and let-7 stRNAs. Our findings suggest that a common processing machinery generates guide RNAs that mediate both RNAi and endogenous gene regulation.
Pubmed ID: 11461699 RIS Download
Mesh terms: Animals | Animals, Genetically Modified | Caenorhabditis elegans | DNA Primers | Drosophila | Embryo, Nonmammalian | Female | Gene Expression Regulation, Developmental | Gene Silencing | Genes, Helminth | Genes, Reporter | Genomic Imprinting | Heterozygote | Larva | Luciferases | Phylogeny | Polymerase Chain Reaction | RNA, Helminth
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.