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Functional analysis of secreted and transmembrane proteins critical to mouse development.

We describe the successful application of a modified gene-trap approach, the secretory trap, to systematically analyze the functions in vivo of large numbers of genes encoding secreted and membrane proteins. Secretory-trap insertions in embryonic stem cells can be transmitted to the germ line of mice with high efficiency and effectively mutate the target gene. Of 60 insertions analyzed in mice, one-third cause recessive lethal phenotypes affecting various stages of embryonic and postnatal development. Thus, secretory-trap mutagenesis can be used for a genome-wide functional analysis of cell signaling pathways that are critical for normal mammalian development and physiology.

Pubmed ID: 11431694

Authors

  • Mitchell KJ
  • Pinson KI
  • Kelly OG
  • Brennan J
  • Zupicich J
  • Scherz P
  • Leighton PA
  • Goodrich LV
  • Lu X
  • Avery BJ
  • Tate P
  • Dill K
  • Pangilinan E
  • Wakenight P
  • Tessier-Lavigne M
  • Skarnes WC

Journal

Nature genetics

Publication Data

July 29, 2001

Associated Grants

None

Mesh Terms

  • Animals
  • Blastocyst
  • Breeding
  • Genes, Lethal
  • Genetic Vectors
  • Genotype
  • Membrane Proteins
  • Mice
  • Molecular Biology
  • Mutagenesis, Insertional
  • Phenotype
  • Polymerase Chain Reaction
  • Proteins
  • Selection, Genetic
  • Sequence Tagged Sites
  • Stem Cells