• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Multiple pathways cooperate in the suppression of genome instability in Saccharomyces cerevisiae.

Gross chromosome rearrangements (GCRs), such as translocations, deletion of a chromosome arm, interstitial deletions and inversions, are often observed in cancer cells. Spontaneous GCRs are rare in Saccharomyces cerevisiae; however, the existence of mutator mutants with increased genome instability suggests that GCRs are actively suppressed. Here we show by genetic analysis that these genome rearrangements probably result from DNA replication errors and are suppressed by at least three interacting pathways or groups of proteins: S-phase checkpoint functions, recombination proteins and proteins that prevent de novo addition of telomeres at double-strand breaks (DSBs). Mutations that inactivate these pathways cause high rates of GCRs and show synergistic interactions, indicating that the pathways that suppress GCRs all compete for the same DNA substrates.

Pubmed ID: 11429610

Authors

  • Myung K
  • Chen C
  • Kolodner RD

Journal

Nature

Publication Data

June 28, 2001

Associated Grants

None

Mesh Terms

  • DNA Damage
  • DNA Helicases
  • DNA Repair
  • DNA Replication
  • DNA, Fungal
  • Fungal Proteins
  • Gene Rearrangement
  • Genes, cdc
  • Genome, Fungal
  • Mutation
  • Recombinant Proteins
  • S Phase
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Telomerase
  • Telomere
  • Translocation, Genetic