We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver.

Science (New York, N.Y.) | May 25, 2001

Mature erythrocytes in mammals have no nuclei, although they differentiate from nucleated precursor cells. The mechanism by which enucleation occurs is not well understood. Here we show that deoxyribonuclease II (DNase II) is indispensable for definitive erythropoiesis in mouse fetal liver. No live DNase II-null mice were born, owing to severe anemia. When mutant fetal liver cells were transferred into lethally irradiated wild-type mice, mature red blood cells were generated from the mutant cells, suggesting that DNase II functions in a non-cell-autonomous manner. Histochemical analyses indicated that the critical cellular sources of DNase II are macrophages present at the site of definitive erythropoiesis in the fetal liver. Thus, DNase II in macrophages appears to be responsible for destroying the nuclear DNA expelled from erythroid precursor cells.

Pubmed ID: 11375492 RIS Download

Mesh terms: Animals | Apoptosis | Cell Differentiation | Cell Transplantation | DNA | DNA-Binding Proteins | Endodeoxyribonucleases | Erythroblasts | Erythroid Precursor Cells | Erythropoiesis | Fetus | Gene Targeting | Globins | Hematopoiesis, Extramedullary | Kruppel-Like Transcription Factors | Liver | Lysosomes | Macrophages | Mice | Mice, Knockout | Mutation | RNA, Messenger | Transcription Factors

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants


Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.