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Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins.

Synaptotagmins (Syt), rabphilin-3A, and Doc2 belong to a family of carboxyl terminal type (C-type) tandem C2 proteins and are thought to be involved in vesicular trafficking. We have cloned and characterized a novel family of C-type tandem C2 proteins, designated Slp1-3 (synaptotagmin-like protein 1-3). The Slp1-3 C2 domains show high homology to granuphilin-a C2 domains, but the amino-terminal domain of Slp1-3 does not contain any known protein motifs or a transmembrane domain. A subcellular fractionation study indicated that Slp1-3 proteins are peripheral membrane proteins. Phospholipid binding experiments indicated that Slp3 is a Ca(2+)-dependent isoform, but Slp1 and Slp2 are Ca(2+)-independent isoforms, because only the Slp3 C2A domain showed Ca(2+)-dependent phospholipid binding activity. The C-terminus of Slp1-3 also bound neurexin Ialpha in vitro, in the same manner as Syt family proteins, which may be important for the membrane association of Slp1-3. In addition, Slp family proteins are differentially distributed in different mouse tissues and at different developmental stages.

Pubmed ID: 11243866

Authors

  • Fukuda M
  • Mikoshiba K

Journal

Biochemical and biophysical research communications

Publication Data

March 13, 2001

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Calcium-Binding Proteins
  • Cloning, Molecular
  • Dimerization
  • Glycoproteins
  • Intracellular Membranes
  • Membrane Glycoproteins
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Neuropeptides
  • Phospholipids
  • Phylogeny
  • Protein Structure, Tertiary
  • RNA, Messenger
  • Sequence Homology, Amino Acid
  • Synaptotagmins
  • Transfection