• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins.

Synaptotagmins (Syt), rabphilin-3A, and Doc2 belong to a family of carboxyl terminal type (C-type) tandem C2 proteins and are thought to be involved in vesicular trafficking. We have cloned and characterized a novel family of C-type tandem C2 proteins, designated Slp1-3 (synaptotagmin-like protein 1-3). The Slp1-3 C2 domains show high homology to granuphilin-a C2 domains, but the amino-terminal domain of Slp1-3 does not contain any known protein motifs or a transmembrane domain. A subcellular fractionation study indicated that Slp1-3 proteins are peripheral membrane proteins. Phospholipid binding experiments indicated that Slp3 is a Ca(2+)-dependent isoform, but Slp1 and Slp2 are Ca(2+)-independent isoforms, because only the Slp3 C2A domain showed Ca(2+)-dependent phospholipid binding activity. The C-terminus of Slp1-3 also bound neurexin Ialpha in vitro, in the same manner as Syt family proteins, which may be important for the membrane association of Slp1-3. In addition, Slp family proteins are differentially distributed in different mouse tissues and at different developmental stages.

Pubmed ID: 11243866


  • Fukuda M
  • Mikoshiba K


Biochemical and biophysical research communications

Publication Data

March 13, 2001

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Calcium-Binding Proteins
  • Cloning, Molecular
  • Dimerization
  • Glycoproteins
  • Intracellular Membranes
  • Membrane Glycoproteins
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Neuropeptides
  • Phospholipids
  • Phylogeny
  • Protein Structure, Tertiary
  • RNA, Messenger
  • Sequence Homology, Amino Acid
  • Synaptotagmins
  • Transfection