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Ten1 functions in telomere end protection and length regulation in association with Stn1 and Cdc13.

The EMBO journal | Mar 1, 2001

In Saccharomyces cerevisiae, Cdc13 has been proposed to mediate telomerase recruitment at telomere ends. Stn1, which associates with Cdc13 by the two-hybrid interaction, has been implicated in telomere maintenance. Ten1, a previously uncharacterized protein, was found to associate physically with both Stn1 and Cdc13. A binding defect between Stn1-13 and Ten1 was responsible for the long telomere phenotype of stn1-13 mutant cells. Moreover, rescue of the cdc13-1 mutation by STN1 was much improved when TEN1 was simultaneously overexpressed. Several ten1 mutations were found to confer telomerase-dependent telomere lengthening. Other, temperature-sensitive, mutants of TEN1 arrested at G(2)/M via activation of the Rad9-dependent DNA damage checkpoint. These ten1 mutant cells were found to accumulate single-stranded DNA in telomeric regions of the chromosomes. We propose that Ten1 is required to regulate telomere length, as well as to prevent lethal damage to telomeric DNA.

Pubmed ID: 11230140 RIS Download

Mesh terms: Alleles | Cell Cycle | Cell Cycle Proteins | Chromosomal Proteins, Non-Histone | Cyclin B | DNA Damage | DNA-Binding Proteins | Flow Cytometry | Fungal Proteins | Genes, Essential | Models, Biological | Mutation | Phenotype | Precipitin Tests | Protein Binding | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Telomerase | Telomere | Two-Hybrid System Techniques

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