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The C-terminus of human glutaminase L mediates association with PDZ domain-containing proteins.

The enzyme glutaminase in brain is responsible for the synthesis of neurotransmitter glutamate. We used the two-hybrid genetic selection system in yeast to look for interactors of glutaminase in human brain. We have identified two proteins containing PDZ domains, alpha1-syntrophin and a glutaminase-interacting protein, named GIP, that showed association with human glutaminase L, as deduced from specificity test of the two-hybrid system. The complete GIP cDNA clone has 1315 nucleotides with a 372-base open reading frame encoding a 124-amino acids protein. Glutaminase associates with both PDZ proteins through its C-terminal end; mutagenesis of single amino acids revealed the sequence -ESXV as essential for the interaction. These data suggest the possibility that PDZ domain-containing proteins are involved in the regulation of glutaminase in brain.

Pubmed ID: 11163757

Authors

  • Olalla L
  • Aledo JC
  • Bannenberg G
  • Márquez J

Journal

FEBS letters

Publication Data

January 19, 2001

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • Brain
  • Calcium-Binding Proteins
  • Glutaminase
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Isoenzymes
  • Membrane Proteins
  • Molecular Sequence Data
  • Muscle Proteins
  • Mutation
  • Neurotransmitter Agents
  • Protein Binding
  • Protein Structure, Tertiary
  • Sequence Alignment
  • Two-Hybrid System Techniques