Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Dexras1: a G protein specifically coupled to neuronal nitric oxide synthase via CAPON.

Neuron | Oct 21, 2000

Because nitric oxide (NO) is a highly reactive signaling molecule, chemical inactivation by reaction with oxygen, superoxide, and glutathione competes with specific interactions with target proteins. NO signaling may be enhanced by adaptor proteins that couple neuronal NO synthase (nNOS) to specific target proteins. Here we identify a selective interaction of the nNOS adaptor protein CAPON with Dexras1, a brain-enriched member of the Ras family of small monomeric G proteins. We find that Dexras1 is activated by NO donors as well as by NMDA receptor-stimulated NO synthesis in cortical neurons. The importance of Dexras1 as a physiologic target of nNOS is established by the selective decrease of Dexras1 activation, but not H-Ras or four other Ras family members, in the brains of mice harboring a targeted genomic deletion of nNOS (nNOS-/-). We also find that nNOS, CAPON, and Dexras1 form a ternary complex that enhances the ability of nNOS to activate Dexras1. These findings identify Dexras1 as a novel physiologic NO effector and suggest that anchoring of nNOS to specific targets is a mechanism by which NO signaling is enhanced.

Pubmed ID: 11086993 RIS Download

Mesh terms: Adaptor Proteins, Signal Transducing | Animals | Carrier Proteins | Cells, Cultured | Cerebral Cortex | Cyclic GMP | GTP-Binding Proteins | Guanine Nucleotides | Mice | Mice, Knockout | Molecular Sequence Data | Monomeric GTP-Binding Proteins | Neurons | Nitric Oxide | Nitric Oxide Donors | Nitric Oxide Synthase | Nitric Oxide Synthase Type I | Organ Specificity | Protein Binding | RNA, Messenger | Rats | Receptors, N-Methyl-D-Aspartate | Sulfhydryl Compounds | ras Proteins

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIDA NIH HHS, Id: DA00074
  • Agency: NIMH NIH HHS, Id: MH-18501

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.